DirectLoad™ Wide Range DNA Marker ready-to-use marker for ...

Sigma-Aldrich offers Sigma-D7058, DirectLoad™ Wide Range DNA Marker for your research needs. Find product specific ... For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%. ... Plant Biotech Guide (910 KB ).

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DNA Protocols & Applications - QIAGEN

Spectrophotometric measurement of DNA concentration ... Bacterial plasmids are closed circular molecules of double-stranded DNA that range in size from 1 to >200 .... Disrupted plant material can be stored in a suitable lysis buffer for several...

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Size and Structure of Replicating Mitochondrial DNA ... - The Plant Cell

well and that the largest amount of DNA migrating from the well was present as 50- to .... tion, the size range of the DNA in this region appeared to decrease with...

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Introduction — Plant DNA C-values Database — Royal Botanic ...

The DNA C-value of an organism is the amount of nuclear DNA in its ... Information about C-values is used in a wide range of biological fields (e.g. see Bennett...

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A Comparison of DNA Extraction Methods using Petunia hybrida ...

Extraction of DNA from plant tissue is often problematic, as many plants contain high .... Range of DNA concentrations obtained using the CTAB method and the...

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Laboratory diagnosis of Phytophthora ramorum from field samples

With these "hard-to-culture from" plant species, DNA-based technologies to ... to be highly specific for Pr especially in the range of DNA concentrations one might...

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Detection and Avoidance of Polysaccharides in Plant ... - Nanodrop

both in order to quantify the DNA or RNA, and also to assess purity. By measuring the ... In this study, the effect of salt concentration in extraction buffer on the .... dynamic range and low volume requirements, coupled with the automatic...

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A simple and efficient genomic DNA extraction protocol for large ...

Mar 5, 2015 ... Extraction of high quality genomic DNA from higher plants is hindered ... Our method used SDS and high salt concentrations to extract DNA and does .... plant varieties using a wide range of SSR markers on DNA extracts from...

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Range of Phytoplasma Concentrations in Various Plant Hosts as ...

(DAPI) fluorescence microscopy, a wide range of phytoplasma ..... plasma concentrations in infected plant tissue, 1 µl of sample DNA and a 10-fold serial dilution...

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The Difference of the Genomic DNA Extraction Between Animal ...

The amount and purity of extracted DNA depends on the type and size of the cell, ... in plants such as photosynthesis produce a range of secondary metabolites.

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DNA Protocols & Applications - QIAGEN

Spectrophotometric measurement of DNA concentration ... Bacterial plasmids are closed circular molecules of double-stranded DNA that range in size from 1 to >200 .... Disrupted plant material can be stored in a suitable lysis buffer for several...

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Extraction of DNA From Plants Using Plant DNAzol® Reagent ...

Following extraction, plant debris is removed by centrifugation and DNA is ... In case of contact, wash skin with a copious amount of water; seek medical attention. TOP .... Molecular weight of the isolated DNA ranges from 20 to 100 kb with the...

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Modified CTAB Technique for Isolation of DNA from some Medicinal ...

DNA isolation protocol for genetic characterization of any medicinal plants is the ... genomic DNA with purity range between 1.26 to 1.93 and concentration...

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Phosphorus Uptake by Plants: From Soil to Cell - Plant Physiology

The proportion of the total P in each chemical form (except P in DNA) .... Concentrations of Pi in the xylem range from 1 mm in Pi-starved plants to 7 mm in plants...

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Modified CTAB Technique for Isolation of DNA from some Medicinal ...

DNA isolation protocol for genetic characterization of any medicinal plants is the ... genomic DNA with purity range between 1.26 to 1.93 and concentration...

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PCR Troubleshooting - Microbiology Gateway

The amount of total DNA in a PCR has a marked effect on the outcome of a .... especially fragments in the size range 100bp to 1000bp, a KCl concentration of...

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Thermo Scientific GeneJET Nucleic Acid ... - Fisher Scientific

Concentration. Viral DNA and RNA. Total RNA. Maxiprep. Plant. DNA. Plant. DNA. From. PCR ... M - Thermo Scientific™ FastRuler™ High Range. DNA Ladder...

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Plant DNA Isolation Reagent - Clontech

Takara plant DNA Isolation Reagent is a ready-to-use kit that uses benzyl chloride for extracting plant genomic .... Dry the pellet, and resuspend in a suitable amount of TE buffer (about 20 μl) .... M:Wide-Range DNA Ladder(50 - 10,000 bp).

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A Beginner's Guide to ChIP - Abcam

ChIP using plant samples - Arabidopsis . .... As the proteins are cross-linked to the DNA a broad range of chromatin associated factors can be .... antibody required by performing a ChIP experiment using a range of antibody concentrations.

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Evaluation of methods for DNA extraction from must and wine

methods, isolation of DNA in the appropriate amount and ... plant part and leaf structure, leaf age or chemicals (2). ... A range of sample volumes from must.

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A Rapid and Effective Method for Isolation of Genomic DNA from ...

Jun 26, 2015 ... The traditional lengthy plant DNA isolation protocol by using CTAB detergent ... materials and provides a high yield of DNA in the range of 142.5 μg ... for the isolation of plant genomic DNA from the small amount of silica dried...

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Genome size - Wikipedia, the free encyclopedia

Genome size is the total amount of DNA contained within one copy of a single genome. ... In animals they range more than 3,300-fold, and in land plants they differ by a factor of about 1,000. Protist genomes have been reported to vary more...

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original version - MDPI

Dec 3, 2012 ... The activity of the plant extract and various subfractions against selected ... range of % peroxidation inhibition was 35.42–93.48%. ... induced oxidative damage in plasmid pBR322 DNA was also evaluated, and it was ... Some other compounds found in different concentrations were: β-ocimene (3.82%),.

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QuantiGene Plex DNA CNV Assay-Overview - eBioscience

Compatible with plant and animal tissues, FFPE tissue sections, whole blood, ... The QuantiGene Plex DNA Assay has a 3 log linear working range and can ... into a constant amount of mouse genomic DNA (180 ng or 30,000 cell equivalents,...

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DNA Concentration - ResearchGate

How can I use the DNA concentration obtained from nanodrop to evaluate the .... As a side note, you might want to take a look at the plant chromatin extraction and .... The 260:280 is within range, but the 260 by itself is way too less considering...

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A simplified protocol for the recovery of high quality DNA from nutmeg

The DNA recovery from the tender leaf tissues was high in the mean range of. 83.55 μg/g with a ... original protocols and using same amount of plant material at...

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Purifying Genomic DNA from Plant Tissue on Automated High ...

Promega has developed the Wizard Magnetic 96 DNA Plant System (“Wizard ... Sample concentrations will fall into a range of +/− 50% of the average value as...

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Guidelines for PCR Optimization with Taq DNA Polymerase | NEB

1.5-2.0 mM is optimal for Taq DNA Polymerase; Optimal concentration depends on ... are 5°C below the lowest primer's Tm and often fall in the range of 50-60°C...

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ISOLATE II Plant DNA Kit | bioline

ISOLATE II Plant DNA Kit is designed for the rapid purification of highest quality ... they need to allow high-levels of stable recombinant proteins to be produced that ... Bioline's range of RNA Analysis products are manufactured and packaged...

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Anthocyanins protect DNA from UV damage

Plants use both DNA repair and various shielding strategies to minimize DNA ... Because anthocyanins and other flavonoids absorb light in the UV-B range, it is ... to 1/3 the concentration in K8 plants) are interposed between DNA and a UV...

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Evolution of DNA Amounts Across Land Plants (Embryophyta)

Recent increases in C-value data (e.g. Plant DNA C-values database; release 2.0, January ... of how the genome size (1C nuclear DNA amount) of species may change. ... 2A) contrasts with the very narrow 12-fold range in bryophytes (Fig.

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Ultraviolet Radiation: How It Affects Life on Earth : Feature Articles

The sun radiates energy in a wide range of wavelengths, most of which are invisible ... People and plants live with both helpful and harmful effects of ultraviolet (UV) ... DNA readily absorbs UV-B radiation, which commonly changes the shape of ... the chemistry of the atmosphere in a way that reduces the amount of ozone in...

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RNA - The Genome Analysis Centre

DNA and RNA quality and quantity are the two most critical factors in the success of sequencing projects. ... Sample quantity and concentration for the various NGS platforms ... dsDNA assay (Broad Range or High Sensitivity) or the Pico Green assay (Invitrogen). ... and 7 or above for non-mammalian and plant samples.

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Plant Nutrient Functions and Deficiency and ... - MSU Extension

Module 9 • Plant Nutrient Functions and Deficiency and Toxicity Symptoms and most likely ... range of nutrient amount necessary to meet the ... health and amount of nutrient available. (Brady and .... production of proteins, nucleic acids (DNA.

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Evolution of genome size in the angiosperms

Genome size varies extensively across the flowering plants, which has stimulated .... Despite the enormous range in nuclear DNA amount, most angiosperms...

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DNA extract characterization process for microbial detection ...

DNA extraction DNA characterization DNA concentration DNA purity ... For the A260/A230 ratio the community accepted range is 1.8 to 2.2 [9], ..... downstream detection of plant diseases and pathogen contamination in food and water [36].

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